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Proteinase K, RNase DNase free 1336 21 6 ASTs decrease the risk of 3 12 3483 ASTs decrease the risk of using an antibiotic against bacteria exhibiting resistance to it HEPES interferes with reactions between DNA and restriction enzymes to a lesser extent than similar buffers with less substituted amine groups Unit Definition: One unit is defined as the amount of enzyme that will liberate 1 Proteinase K is inactivated by heating to 95°C for 10 minutes or using an inhibitor such as PMSF
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